Development and Validation of an RP-HPLC Method for Estimation of Cefuroxime Axetil and Its Degradation Products in Tablets
ثبت نشده
چکیده
The absorbed ester is hydrolyzed in the intestinal mucosa and in portal circulation [3]. Estimation of cefuroxime Axetil has been described by spectrophotometry [3-5], HPTLC [6-8] and HPLC [918]. HPLC methods are available in United State Pharmacopoeia [19] and in British pharmacopoeia [1]. The column in the official methods is Trimethylsilane bonded-phase. The mobile phase consisting of 0.2M ammonium dihyrogen phosphate (23 gm/ litre). In the official methods the buffer concentration is very high and that may be lead to adversely affect on the operation of HPLC system. The buffer may be precipitate in the column and LC system when it is exposed to the highest organic concentration. The backpressure is very high.
منابع مشابه
Rapid Analysis of Glibenclamide Using an Environmentally Benign Stability-Indicating RP-HPLC Method
An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...
متن کاملRapid Analysis of Glibenclamide Using an Environmentally Benign Stability-Indicating RP-HPLC Method
An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...
متن کاملA New Stability Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Dolutegravir and Rilpivirine in Bulk and its Dosage Forms
A New Stability Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Dolutegravir and Rilpivirine in Bulk and its Dosage Forms. Abstract The objective of the work is to develop and validate a new, simple, highly sensitive, stability indicating RP-HPLC method for simultaneous estimation of Dolutegravir and Rilpivirine in bulk and its dosage f...
متن کاملThe stability of Cefuroxime axetil in tablets.
The stability of cefuroxime axetil in BIORACEF tablets was studied by means of long term (298 K/60% RH), intermediate (303 K/65% RH), accelerated (313 K/75% RH) and stress stability tests. Changes in the concentration of cefuroxime axetil diastereoisomers A and B and their total was determined using the RP-HPLC method, as described in a monograph of Cefuroxime axetil tablets in the British Phar...
متن کاملDevelopment and validation of a fast, simple and specific stability indicating RP-HPLC method for determination of Dexpanthenol in eye gel formulation.
AIn this study a simple and reliable stability-indicating RP-HPLC method was developed and validated for analysis of Dexpanthenol in an artificial tear formulation. The chromatographic separation was performed on a HPLC C18 column (25.0 cm 4.6 mm, 5 m) using a mixture of 0.037 M monobasic potassium phosphate in water (adjusted with 0.1% (v/v) phosphoric acid to a pH of 3.2) and methanol (90:...
متن کامل